Part:BBa_K3738015
MS2 Bacteriophage Capsid Protein with N-Terminal Histidine Tag (Composite Part)
The MS2 capsid protein comprises most of the capsid of the MS2 phage, as well as MS2 phage-like particles (PLPs). A single MS2 capsid requires 178 copies of the capsid protein, as one copy of maturation protein A (BBa_K3738000), which is critical in allowing the capsid to bind to the cell and deliver its cargo. This composite part includes the IPTG-inducible T7 promoter (BBa_J64997), RBS BBa_B0034, MS2 capsid protein coding region (BBa_K3738005), and double terminator BBa_B0015. The MS2 capsid protein coding region in this part includes a histidine tag on the N-terminus for to allow for peptide purification.
The Lethbridge 2022 Team added experience to this part as a part of their contributions for the Bronze Medal Requirement.
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 485
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 485
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 485
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 485
- 1000COMPATIBLE WITH RFC[1000]
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